Electroporation is a technique that uses electric fields to introduce foreign genetic material into cells by creating temporary pores in the cell membrane. In small animal research, electroporation can be employed both in vitro (cell culture) and in vivo (directly in the animal). Here, we will focus on in vivo electroporation in small animals and…

Viral vectors are engineered viruses that can deliver foreign genetic material into host cells. They are widely used for gene delivery in research and gene therapy applications due to their high transduction efficiency and ability to infect a wide range of cell types. There are several types of viral vectors, each with unique properties, advantages,…

Nanoparticle-mediated transfection is a promising approach for gene delivery in small animals. This method uses nanoparticles, which are nanometer-sized particles made from various materials, to encapsulate or complex with genetic material and deliver it into cells. Nanoparticles can protect the genetic material from degradation, improve cellular uptake, and provide targeted delivery. Materials: Different materials can…

Polymer-based transfection reagents are composed of natural or synthetic polymers that can form complexes with genetic material, such as plasmid DNA, siRNA, or mRNA, to facilitate their delivery into cells. These reagents offer several attractive features for small animal research, which have led to their widespread use in various applications. Features of polymer-based transfection reagents:…

Sonoporation is a non-viral, non-invasive technique that uses ultrasound energy in combination with microbubbles to facilitate the delivery of genetic material into cells. This method has gained interest in small animal research due to its potential for targeted, efficient, and safe gene delivery. Mechanism: Advantages of sonoporation and microbubble-assisted transfection in small animals: Challenges and…

There are several transfection methods available for small animal research, each with their own advantages and disadvantages. Here is a comparison of some of the most commonly used transfection methods: Overall, the choice of transfection method for small animal research depends on the specific experimental requirements, including the desired level and duration of gene expression,…

Small animal models are widely used in cancer research to investigate the mechanisms of tumorigenesis, test potential cancer therapies, and develop new cancer models. Transfection can be used in small animal models to deliver genes, shRNAs, or siRNAs that modulate gene expression or function, allowing researchers to investigate the roles of specific genes in cancer…

Transfection-based vaccines are a promising approach for developing vaccines for small animals. These vaccines typically involve the delivery of DNA or mRNA encoding viral or bacterial antigens into cells, which then express the antigens and elicit an immune response. Here are some key considerations in the development of transfection-based vaccines for small animals: Transfection-based vaccines…

There are several transfection methods available for small animal research, each with their own advantages and disadvantages. Here is a comparison of some of the most commonly used transfection methods: Overall, the choice of transfection method for small animal research depends on the specific experimental requirements, including the desired level and duration of gene expression,…

Small animal models are widely used in cancer research to investigate the mechanisms of tumorigenesis, test potential cancer therapies, and develop new cancer models. Transfection can be used in small animal models to deliver genes, shRNAs, or siRNAs that modulate gene expression or function, allowing researchers to investigate the roles of specific genes in cancer…